منابع مشابه
Phytochrome immunoaffinity purification.
We have developed a phytochrome immunoaffinity purification procedure that yields undegraded oat (Avena sativa L., cv. Garry) phytochrome of greater than 98% purity within 2 hours when starting with a brushite-purified preparation. Immunoaffinity-purified phytochrome, except for its greater purity, is indistinguishable from conventionally purified phytochrome by gel exclusion chromatography, is...
متن کاملImmunoaffinity purification of dietary heterocyclic amine carcinogens.
Cooking meats produces a family of heterocylic aromatic amines that are carcinogens in rodents and genotoxic in many short-term assays. Concern that these compounds may be human carcinogens has prompted us to develop immunochemical methods for quantifying these compounds in the human diet and for identifying the parent compounds and metabolites in urine and feces. Previously reported monoclonal...
متن کاملImmunoaffinity purification of bovine factor VII.
Factor VII has been purified to homogeneity from bovine plasma by a procedure that includes affinity purification on an immunoadsorbent column. Recovery was determined by both coagulant assay and liquid scintillation counting, using 3H-factor VII as an internal standard. The purification factor calculated by both methods was approximately 120,000-fold, with a final yield of approximately 18%. H...
متن کاملPurification of nucleotide-requiring enzymes by immunoaffinity chromatography.
Monospecific (affinity-purified) anti-(yeast glucose-6-phosphate dehydrogenase) IgG inhibits three different NADPH-requiring enzymes, chicken liver dihydrofolate reductase, pigeon liver fatty acid synthetase and chicken liver malic enzyme. The inhibition of all three enzymes was approx. 50% in a 2h incubation with 100 micrograms of IgG. Similarly, with several different NADH-requiring enzymes, ...
متن کامل[10] Protein- and Immunoaffinity Purification of Multiprotein Complexes
We have not had any problems using this method to purify proteins that are lethal on overexpression, including Abplp and Duolp. Although cells are dying during the induction, significant amounts of recombinant protein are produced. Because some proteins are sensitive to longer (>4 hr) induction times, it may be important to test protein expression at different time points during the induction. ...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1979
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.64.2.332